All analysis uses ASTRA (Wyatt’s software for SEC in-line MALS). At the beamline, we use ASTRA 7.
- Open an experiment (your file)
- Open Experiments tab on the bottom left
- Configuration -> generic pump (on left side panel)
- Set flow rate, (default is 0.8 but you should remember to change this to the real flow rate for any given experiment).
- Procedures -> baselines (on left)
- First click autofind baselines
- Click through baselines and make sure all baselines are defined (if they are not good you have to define them; click once at start point and, hold and drag it to right side to define the baseline and range)
- Click Apply
- In the top toolbar, Experiment -> configuration -> band broadening
- Define an area
- Click Perform fit
- Manually define peaks if needed
- Click Apply
- Click Peaks on left side panel
- Choose what peaks (UV curve) you will be working with (better to choose range above baseline)
- Click Apply
- Click Molecular Mass & Radius from LS on left side panel
- De-select detectors that had bad baselines
- Click Apply
- Can scroll through peaks to see MW for each peak from MALS
- Click Rh from QELS on left side panel
- Can scroll through peak and see Rh from QELS (DLS)