BioCAT offered its third MuscleX workshop entitled “Sarcomeric regulation mechanisms in health and disease” from May 18 -19 2023. This entirely virtual workshop had 200 registered attendees. The workshop started with an introductory presentation of the scientific missions supported at BioCAT followed by a series of 13 scientific presentations highlighting recent muscle studies using x-ray diffraction and/or other structural techniques. Topical areas that were covered were 1) Thick filament-based regulation, 2) New insights into thin filament regulation, 3) Titin and muscle regulation, 4) MyBPC and regulation of thick and thin filament, and 5) Translational studies on structural bases of muscle diseases. Speakers came from several US states, the UK, Italy and Germany. Of the 13 speakers, eight of them were at an early career stage. Speakers included Thomas Irving (BioCAT, APS, USA), Weikang Ma (BioCAT, APS, USA), Yanhong Wang (King’s College London, UK), Ilaria Morotti (U Florence, Italy) , Saffie Mohran (University of Washington, USA),Neil Kad (University of Kent, UK), Davide Tamborrini (Max Planck Institute of Molecular Physiology, Germany), Anthony Hessel (University of Muenster, Germany), Caterina Squarci (U Florence, Italy), Maicon Landim-Vieira (Florida State University, USA), Samantha Harris (U Arizona Tucson, USA), Vivek Jani (Johns Hopkins University, USA), Farid Moussavi- Harami (University of Washington, USA).
Some of the lectures were recorded and are available on the BioCAT YouTube channel:
- Welcome and introduction to scientific missions at BioCAT by Thomas Irving
- Thick filament regulation in porcine myocardium by Weikang Ma
- The structurally-defined OFF and ON state can be decoupled with biochemically-defined super-relaxed and disordered-relaxed state by Saffie Mohran
- Single-molecule imaging of thick filament regulation in myofibrils by Neil Kad
- In situ structures from relaxed cardiac myofibrils reveal the organization of the muscle thick filament by Davide Tamborrini
- Titin activates myosin filaments in skeletal muscle by switching from an extensible spring to a mechanical rectifier by Caterina Squarci
- Cardiac Troponin T N-domain variant destabilizes the actin interface resulting in disturbed myofilament function by Maicon Landim-Vieira
- Minding the Gap: Myosin Binding Protein C steps over the Interfilament Space by Samantha Harris
- Right Ventricular Cardiomyocyte Sarcomere Dysfunction and Deficient Thick Filament Activation in Human Heart Failure with Right Ventricular Dysfunction by Vivek Jani
- Myosin activator Danicamtiv increases myosin recruitment and alters the chemomechanical cross bridge cycle in cardiac muscle by Farid Moussavi-Harami