BioCAT is offering its fifth intensive HOW-TO course in BioSAXS. Students will have two days of lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. This will be followed by data collection on the BioCAT beamline (Sector 18 at the APS) and data analysis help. Students are encouraged to bring 1-2 research samples for the data collection.
The course will take place from 11/5/19-11/7/19 at the APS (see schedule below for details).
For the first time ever, BioCAT is offering this course for remote participants. Remote participants will get a live-streamed feed of the lectures and all of the tutorial materials. They will not be able to collect data.
Registration types and cost
BioCAT is offering two methods of participation for this workshop. The first is on-site, the second is remote.
On-site
The on-site participant registration includes the lectures, hands-on training, and data collection. Lunch and coffee breaks are provided. Due to time constraints on data collection we can only accept 15 on-site participants.
On-site cost: $150/$300 for academic/industrial
Remote
Remote participant registration provides live-streams of all the lectures and downloads of all course materials, including lecture slides and tutorials. The tutorials are designed to be self-guided and so can be completed by the remote participants, but there is no live-stream of that portion. No data collection is associated with remote participation.
Remote cost: $50/$100 for academic/industrial
How to register
Lodging and Travel
On-site participants are responsible for their own lodging and travel. We recommend participants plan to arrive Monday (11/4) and leave Friday morning (11/8). Rooms are available at the Argonne Guest House.
More information
Course topics:
- Basic principles of SAXS
- Critical sample preparation and data collection procedures, including online size-exclusion chromatography (SEC-SAXS)
- Evaluating data quality
- Basic data processing including size (Rg, Dmax), molecular weight, the P(r) function, and real space reconstructions
- What you need to know for publication
- Overview of several advanced and emerging methods
- Hands-on tutorials with data processing software
- Hands-on data collection and analysis at the beamline
While the primary purpose of the course is educational, on-site participants may bring a limited number (1-2) of their own research samples. Standard protein samples will be available to all on-site participants for training purposes.
Participants will be assigned into small groups for 4 hour data collection slots starting Wednesday afternoon and running through Thursday evening (11 pm). If you are coming as a group or if you need a particular time slot, please notify Jesse Hopkins (jhopkins1@iit.edu) for scheduling. Data analysis help is available in parallel with the data collection on Thursday, but it is not a formal tutorial.
While BioCAT supports SEC-SAXS, SEC-MALS-SAXS, and batch mode SAXS, due to time constraints students will be limited to only SEC-SAXS data collection. Please read our guide on how to design a SAXS experiment and how to prepare your SAXS samples.
If you have questions, please contact Jesse Hopkins (jhopkins1@iit.edu).
Confirmed speakers:
- Jesse Hopkins (BioCAT, APS)
- Srinivas Chakravarthy (BioCAT, APS)
- Xiaobing Zuo (Sector 12, APS)
- Tobin Sosnick (U. Chicago)
- Todd Washington (U. Iowa)
- Kushol Gupta (U. Pennsylvania)
Tentative schedule
Tuesday 11/5/19
Note: All of Tuesday is lectures and will be live-streamed for remote participants.
| 08:00 am | Registration |
| 08:30 am | SAXS overview: what it is and why you should do it |
| 09:00 am | Planning and performing SAXS experiments I: In your lab |
| 09:45 am | Planning and performing SAXS experiments II: At the beamline |
| 10:15 am | Planning your (second) BioCAT experiment |
| 10:30 am | Break |
| 10:50 am | The scattering profile |
| 11:05 am | Basic data analysis: Generating a scattering profile, Rg, MW, Kratky, P(r), and when it all goes wrong |
| 12:15 pm | Group photo |
| 12:20 pm | Lunch (provided) |
| 01:00 pm | Advanced data analysis: SEC-SAXS, reconstructions (DAM, ED), fitting (Crysol/FoXs) |
| 02:00 pm | Publishing your data: best practices |
| 02:30 pm | Advanced topics 1: Time-resolved SAXS |
| 03:15 pm | Break |
| 03:35 pm | Advanced topics 2: Modeling the conformational flexibility of DNA repair proteins using full-ensemble hybrid methods |
| 04:20 pm | Advanced topics 3: Mixtures and complexes |
| 05:05 pm | Preparation for student beamtime |
| 06:45 pm | End of Tuesday lectures |
Wednesday 11/6/19
Note: Only the Advanced Topics 4 lecture will be live-streamed on Wednesday. The tutorials and software introductions will not be. This is the last live-stream of the workshop.
| 08:30 am | BioXTAS RAW introduction and software demo |
| 09:00 am | Hands on with RAW: Basic data processing tutorial |
| 10:00 am | Advanced topics 4: Flexible systems and intrinsically disordered proteins |
| 11:00 am | Hands on with RAW: Basic data processing tutorial |
| 12:30 pm | Lunch (provided) |
| 01:30 pm | BioXTAS RAW software demo |
| 02:00 pm | Hands on with RAW: Advanced data processing tutorial |
| 02:00 pm | Group 1 data collection (Tutorial continues in parallel) |
| 06:00 pm | End of Wednesday tutorials |
| 07:00 pm | Group 2 data collection |
| 11:00 pm | End of Wednesday data collection |
Thursday 11/7/19
Note: There is no live-stream on Thursday.
| 09:00 am | Group 3 data collection |
| 01:00 pm | Lunch (not provided) |
| 02:00 pm | Group 4 data collection |
| 06:00 pm | Dinner (not provided) |
| 07:00 pm | Group 5 data collection |
| 11:00 pm | End of Thursday data collection |
Additionally on Thursday, data analysis help will be available from 9 am to 6 pm, except during lunch. Work on the tutorials continues for anyone who didn’t finish it on Wednesday, including those in the Group 1 data collection time.