BioCAT is offering its seventh intensive HOW-TO course in BioSAXS. Students will have four days of virtual lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. Students will also be able to mail in samples for data collection on the BioCAT beamline (Sector 18 at the APS) before the course, and there will be time during the workshop to get help with analysis of their own data.
The course will take place from 3/29/21 to 4/1/21 and is entirely virtual (via BlueJeans). See the schedule below for details.
Registration
All participants this year will be remote participants. The course will provide a virtual set of lectures and tutorials via BlueJeans, and participants will be able to mail in samples for SAXS data collection at BioCAT the week before the workshop. Participants will be able to ask questions during both lectures and tutorials, and will have time to get help with analysis of the data collected from their mail-in samples. They will also receive downloads of all course materials, including lecture slides and tutorials.
There is no cost associated with the workshop, but registration is limited to 15 participants, in order to allow for data collection and one-on-one and/or small-group interactions between participants and instructors. Registration is on a first-come first-served basis.
How to register
More information
Course topics:
- Basic principles of SAXS
- Critical sample preparation and data collection procedures, including online size-exclusion chromatography (SEC-SAXS)
- Evaluating data quality
- Basic data processing including size (Rg, Dmax), molecular weight, the P(r) function, and real space reconstructions
- What you need to know for publication
- Overview of several advanced and emerging methods
- Hands-on tutorials with data processing software
While the primary purpose of the course is educational, participants may mail-in a limited number (1-2) of their own research samples for data collection the week before the course.
While BioCAT supports SEC-SAXS, SEC-MALS-SAXS, and batch mode SAXS, due to time constraints students will be limited to only SEC-SAXS data collection. Please read our guide on how to design a SAXS experiment and how to prepare your SAXS samples.
If you have questions, please contact Jesse Hopkins (jhopkins1@iit.edu).
Confirmed speakers:
- Jesse Hopkins (BioCAT, APS)
- Srinivas Chakravarthy (BioCAT, APS)
- Richard Gillilan (MacCHESS, CHESS)
- Kushol Gupta (U. Pennsylvania)
- Steve Meisburger (Cornell U.)
- Javier Perez (SWING, Synchrotron SOLEIL)
- Tobin Sosnick (U. Chicago)
Tentative schedule
Note: All times are Central Daylight Time (UTC-5)
Monday 3/29/21
| 10:00 am | Welcome |
| 10:05 am | SAXS overview |
| 10:35 am | SAXS Instrumentation |
| 10:55 am | Planning and performing SAXS experiments (inc. complementary methods) |
| 12:10 am | Break |
| 12:40 pm | Basic SAXS data validation and analysis |
| 01:55 pm | Q&A panel |
| 02:45 pm | End |
Tuesday 3/30/21
| 10:00 am | Overview of SAXS data processing software |
| 10:15 am | Tutorial: Basic data analysis |
| 12:45 pm | SEC-SAXS data collection and analysis |
| 01:15 pm | Tutorial: SEC-SAXS data analysis |
| 02:45 pm | End |
Wednesday 3/31/21
| 10:00 am | Advanced analysis: Reconstructions and model fitting |
| 10:45 am | Publishing your SAXS data |
| 11:00 am | Time resolved SAXS |
| 11:45 am | Tutorial: Reconstructions and model fitting |
| 02:15 pm | End |
Thursday 4/1/21
| 10:00 am | Advanced analysis: Introduction to rigid body modeling |
| 10:45 am | Advanced analysis: Introduction to flexibility and ensemble modeling |
| 11:30 am | Advanced topic: SAXS and membrane proteins |
| 12:15 am | Break |
| 12:45 pm | Advanced topic: SAXS and intrinsically disordered proteins |
| 01:30 pm | Tutorial: Data analysis of participant’s data |
| 02:30 pm | Planning experiments at BioCAT |
| 02:45 pm | Wrap up and final Q&A |
| 03:15 pm | End |