BioCAT supports a number of techniques for analysis of partially-ordered and disordered biological materials. Below is a set of HOWTOs and other guides and tutorials for experiments at the BioCAT facility. These are intended both for new users as they design their experiments and for users at the beamlines as they run their experiments.
These descriptions should help you to decide if our facility can support your research. However, it is strongly recommended that—-prior to visiting BioCAT—- you discuss your experiment with the User Liaison for your technique after reading these descriptions. In this way, your experiment can be tailored to our facility so that you spend less time assembling and debugging your set-up and more time taking high-quality data.
How to Guides
Small- and Wide-Angle Fiber-Diffraction (includes muscle diffraction)
Macromolecular Small-Angle Solution Scattering (SAXS)
- How to design an equilibrium SAXS experiment
- How to prepare your sample for equilibrium SAXS
- How to design a time-resolved (TR) SAXS experiment
- How to collect SEC-SAXS data at the beamline
- How to collect SEC-MALS-SAXS data at the beamline
- How to analyze SAXS data at the beamline
- How to analyze MALS data at the beamline
Tutorials
Everything BioSAXS Workshop Videos and Slides
- Video: Introduction to SAXS (get intro slides)
- Video: SAXS Sample Preparation (get sample prep slides)
- Video: Basic SAXS data analysis and validation (get basic analysis slides)
- Video: SEC-SAXS (get SEC-SAXS slides)
- Video: 3D reconstructions and model fitting of SAXS data (get 3D reconstruction slides)
- Video: SEC-SAXS and advanced SAXS analysis (get advanced analysis slides)
- Video: Publishing SAXS data (get publishing slides)
- Video: Analyzing Flexible and Disordered Macromolecules with SAXS (get flexibility slides)
- Video: Rigid Body Modeling with SAXS data (get rigid body modeling slides)
- Video: Using Molecular Dynamics (MD) to Interpret SAXS Data (get MD-SAXS slides)
- Video: Time Resolved SAXS (get TR-SAXS slides)
- Video: Time Resolved SAXS of Liquid-Liquid Phase Separation (LLPS) (get TR-SAXS of LLPS slides)
- Video: Using SAXS to study biomolecular phase separation (get phase separation slides)
- Video: SAXS on Intrinsically Disordered Proteins (IDPS) (get IDP slides)
- Video: SAXS on Membrane Proteins (get membrane protein slides)
- Video: Small Angle Neutron Scattering (SANS) (get SANS slides)
- Video: How to get SAXS Beam Time at BioCAT (get SAXS beam time slides)